Investigation of The Ovarian Histological Changes af-ter Treatment with Ethephon in Mice

Background: Ethephon including illegal chemicals uses in agricultural production, which is set to accelerate growth and achieve, improve the quality of the product. The most impor-tant side effects of ethephon on the body include cancer, liver disease, kidney disease, reproductive system dysfunction and infertility. The purpose of the study was to investigate the effect of ethephon on ovarian histological changes in mice.Materials and Methods: In this study, 50 adult female mice with an average weight of 30 g were used for the experiments. Animals were divided into five groups respectively: control (without receiving any substance), sham (receiving daily serum physiology orally), ethephon 1 (receiving daily 192 mg/kg oral-ly), ethephon 2 (receiving daily 240 mg/kg orally), ethephon 3 (receiving daily 480 mg/kg). After 21 days, ovaries were sepa-rated for histological studies. Ovaries were embedded in paraf-fin and cut into sections 6mm thick. The sections were stained with Hematoxylin-Eosin (HE) and observed under an optical microscope.Results: The results indicated that in the average dose (240mg/ kg) and high dose (480mg/kg), the follicular atresia increased and hyperemia in different parts of the ovary especially in me-dial portion of the ovary was seen. In high doses, the most atre-sia was seen in the secondary follicles as a cavity formation.Conclusion: It seems that oral administration of ethephon with effect on ovaries can have a deleterious effect on fertility and embryo development in mice.

کلیدواژه ها:

Ovarian follicle ، Atresia ، Histological Changes ، Eth-ephon ، Mice