Beneficial Effect of Platelet-Rich-Plasma on Testicu-lar Ischemia-Reperfusion Injury in Adult Mice

Background: Testicular Torsion (TT) is a common syndrome that could lead to function loss and infertility in the testis. Is-chemia due to testicular torsion and reperfusion related to the detorsion can cause different morphological changes in tes-ticular tissue. In addition, reperfusion induces increased tissue damage after ischemia. Therefore, testicular ischemia and sub-sequent reperfusion (I/R) can lead to testicular tissue damage.Platelet Rich-Plasma (PRP) may has therapeutic effect due to the presence of growth factors. In this study, PRP was used to evaluate its effects.Materials and Methods: Thirty adult male NMRI mice were randomly divided into three equal groups. The first group, is the healthy control, without testicular twisting. Group II: is-chemia - reperfusion (I/R) + Pbs (phosphate-buffer-solution) group, Group III: The I/R + PRP group. In these two groups spermatic cord were twisted for one hour, and after detorsion, Pbs and PRP were injected into the rete testis, respectively. 35 days after surgery, left testis sampled for histomorphometrical examinations. After fixation in 10% formal saline, paraffin sec-tions were prepared and stained with hematoxylin-eosin. Ob-tained data were statistically compared by ANOVA and post hoc-Tukey test (P <0.05).Results: Cross-sectional area, the number of tubules and nu-merical density of the seminiferous tubules were significantly decreased in the I/R-Pbs group compared to the other groups (P<0.05). I/R caused a significant increase in interstitial space of testicular tissue in I/R group compared to the control and I/ R+ PRP groups (P<0.05). PRP could reduce these tissue chang-es compared to the I/R group(P<0.05).Conclusion: PRP is effective for the prevention of testicular torsion damage in mice testis. It seems that, PRP due to posses-sion ample of growth factors, capable to relatively improve the undesirable effects of I/R.

کلیدواژه ها:

Ischemia ، Reperfusion ، Platelet-Rich-Plasma ، Testis ، Torsion