The Effect of L-Arginine on The Ovarian Histology in Mouse Model of the Endometriosis

Background: One of the most common diseases among wom-en is endometriosis, which affects women at the age of fertility. The cause of infertility in women with endometriosis may be due to anatomical problems that lead to adhesions and fibro-sis, hormonal or immune system problems. Endometriosis also causes complications such as ovarian disorders. Based on the antioxidant and anti-inflammatory effects of L-arginine, it can be a solution to reduce the malignant effects of endometriosis on the ovaries. The purpose of this study is to investigate the effect of L-arginine on the ovarian histology in mouse model of endometriosis.Materials and Methods: At the first step, induction of endo-metriosis in mice was done by injection of suspension of endo-metrium separated from the uterus of donor mice. In the second step, the control group was divided into four treatments: control without any treatment, control receiving L-arginine intrapri-tonally (250 mg/1kg, every day), control receiving danazol in-trapritoneally (25 mg/1kg, every other day) and control receiv-ing L-arginine together with danazol. The endometriosis group was divided into four treatments: endometriosis without any treatment, endometriosis receiving L-arginine intrapritoneally (250mg/1kg, every day), endometriosis receiving danazol in-trapritoneally (25mg/1kg, every other day), and endometriosis receiving L-arginine together with danazol. At the end of the treatment period, the mice were dissected then ovaries were separated and histologically examined.Results: Microscopic studies showed that the endometriosis symptoms was reduced in the endometriosis group which re-ceiving L-arginine and simultaneous L-arginine and danazol by increasing ovulation, increasing corpus luteum formation, decreasing the number of ovarian cysts and improvement in the status of the cortex and stroma.Conclusion: According to our findings, danazol and l-arginine can increase the quality of oocytes and induces ovulation. In addition, because of its antioxidant properties, l-arginine can reduce the damages caused by oxidative stress in this disease and thus help to compensate tissue changes induced by endo-metriosis. L-arginine also causes follicular growth and ovula-tion, leads to increasing fertility rates.

کلیدواژه ها:

Ovary ، Endometriosis ، L-Arginine ، Mouse