What Is The Role of PFD in Male Unexplained Infertil-ity and its Treatments

Introduction of sperm function test changes the approach to male infertility and subfertility. Semen analysis, once consid-ered as a fundamental test in male fertility assessment, has been supplanted with sperm function test in special cases(varicocele, recurrent pregnancy loss or assisted reproduction technique (ART) failure, etc.). Since increased sperm DNA injury can re-duce the success rate of assisted reproduction techniques (ART) outcome(Panner Selvam and Agarwal, 2018), strategies for ob-taining sperm with better DNA integrity seem to be necessary. In our center, such strategies have been categorized into three subgroups based on the day of ejaculation.Pre-sampling methods include treatment of underlying causes which may disturb sperm DNA integrity (varicocele, infection, etc) or empirical antioxidant medical therapy. Sampling meth-ods include changing the way to obtain the samples such as short abstinence and testicular sperm. Post-ejaculation methods include advanced and sometimes expensive technologies for sperm processing, which may result in sperm with better DNA integrity. These technologies can be categorized as 1-complex-expensive techniques such as MACS magnetic activated cell sorting), physiologic intracytoplasmic sperm injection (PICSI), intracytoplasmic morphology selected sperm injection (IMSI), etc. which may not be available in the majority of androl-ogy clinics. 2- cost-effective simpler methods such as Zeta potential,micro fluid,etc., which can be set up easily in most infertility laboratory and ART wards. Besides the complex-ity and cost of these newer methods, there is also controversy about their efficacy on sperm DNA integrity and ARToutcomes. In this session we will talk about these strategies as an step wiseapproach in male factor infertility management.