Peripheral Blood Stem Cell Mobilization Can Be Affected by SNS Neurotransmitters

Background: Hematopoietic stem cell transplantation (HSCT) has provided considerable therapeutic success for hematologic and non-hematologic diseases. Improving the efficacy of stem cell mobilization is an essential step in the prosperity of the transplantation process. Granulocyte colony-stimulating fac-tor (G-CSF) is the most common mobilizer agent. However, the significant poor mobilize population is a convincing reason to conduct investigations into another mechanism to enhance mobilization yields. Recently, accumulating evidence has dem-onstrated the involvement of the nervous system in HSC mobi-lization. Therefore, the goal of this study is figuring out the role of donor pre-apheresis epinephrine (EPI) and norepinephrine (NE) levels in mobilization.Materials and Methods: 20 healthy, patient-related donors were included in this prospective study. Donors treated with subcutaneous G-CSF for 4 to 6 days. Blood was collected be-fore G-CSF treatment and before apheresis. ELISA was per-formed for detection of EPI and NE levels in plasma. CD34+ and CD3+ cells were counted by flow cytometry (Attune NxT,Country) with PE-conjugated human anti-CD34 (EXBIO, Czech Republic) and FITC-conjugated human-CD3 (Beckman Coulter). Statistical analyses were performed using SPSS for Windows (Version 19) (SPSS Inc., Chicago, IL, USA) to evalu-ate a correlation between neurotransmitter levels and CD34+ cell count in PB.Results: Measurement of plasma levels of NE and EPI before the start of G-CSF administration was within the normal limits. The levels of CD34+ cells in PB before apheresis and apheresis product were significantly correlated with plasma levels of cat-echolamines. Data showed healthy donors with a higher con-centration of NE and EPI have more CD34+ cells in peripheral blood. Thus, the yield of apheresis is better (P-value: < 0.05). However, the number of CD3+ cells in the apheresis product was not statistically significant (P value: 0.35).Conclusion: The regulatory effects of the nervous system on HSC mobilization is well-defined and has been entered in the clinical setting. Moreover, given that the number of CD3+ cells was not affected by plasma levels of catecholamines, these neu-rotransmitters can be considered as a good HSC mobilizer in the setting of allogeneic HSCT to maximize the usefulness and limit any possible complications of mobilization.

کلیدواژه ها:

Epinephrine ، Norepinephrine ، Mobilization ، G-CSF