Fabrication of a Polysaccharides Base Porous Sub-strate in Myocardial Tissue Engineering

Background: Application of principles and approaches of en-gineering and life sciences to perceive fundamental and mutual structure–function relationships in native and pathological heart tissues resulting from cardiovascular diseases such as myocar-dial infarction and its subsequent side effect including heart failure,and to develop biological substitutes to restore, maintain, or improve tissue function were all emergence objectivity of the term myocardial tissue engineering .From all approaches have been applied in this field so far, deriving of extracellular matrix (ECM) from different native tissue was a novel approach.Materials and Methods: By consideration of this trend, we acellularized an animal myocardial tissue by sodium dodecyl sulphate (SDS, 1%). Its resultant fine pulverized extracellular matrix (ECM) was digested and blended with two polysaccha-ride-based polymers, freeze dried, and fabricated a ternate sub-strate for cell seedingResults: Microscopically images of histological Characteriza-tion of extracellular matrix by Hematoxylin and Eosin, Mas-son’s Trichome, and Alcian blue staining validated the removal of cellular content and preservation of collagen – the most am-ple component present in the ECM – and glycosaminoglycans (GAGS). Evaluation tests including physical, chemical and mechanical characterization of this substrate were performed at in vitro condition. Physical characterization such as morphol-ogy seen by scanning electron microscopy (SEM) and porosity showed porous substrate with interconnected pores and porosi-ty more than 90%. Fourier Transform Infrared (FTIR) spectrum revealed interaction of the present functional groups of applied polysaccharide-based polymers together with digested extracel-lular matrix . Mechanical properties showed that with addition of polysaccharide-based polymers to the composition,unilateral ultimate tensile strength of substrates in comparison with the substrate free from polymers has significantly increased .Seed-ed human pelvic mesenchymal stem cells on the substrates had attachment and proliferation at defined time 1, 3 and 7 days Conclusion: Generally,validated substrate requirements which are including to be porous structure with interconnected pores to facilitate inward and outward transportations of materials, to be biocompatible, and to have suitable mechanical property, made this substrate as a suitable matrix for subsequent hopeful application in myocardial tissue engineering.

کلیدواژه ها:

ECM ، Substrate ، Polysaccharides ، Myocardial Tissue Engineering