Effect of human Adipose Stem Cell Derived Ex-osomes on Proliferation and Neural Differentiation of PC12 cells

Background: The nervous system has limited regeneration capacity. So researchers are always looking for an appropriate treatment for neurodegenerative diseases. Exosome treatment improves the therapeutic outcomes and prevents the concerns of cellular and surgical therapies that are due to the capability of exosomes to start the internal repairing processes of damaged tissues and modulate the immune responses. The aim of this study was to investigate the effect of human Adipose Stem Cell derived exosomes on proliferation and neural differentiation of PC12 cells.Materials and Methods: Following the isolation of hASCs ex-osomes, TEM, DLS, Bradford and Flow cytometry techniques were used to confirm successful isolation. These exosomes were used for proliferation and differentiation of PC12 cells. Entrance and effect of exosomes assessed by Acridine orange staining, MTT assay and ICC techniqueResults: This study present that Adipose Stem Cell derived ex-osomes can enhance cell viability of PC12 cells and promote neural differentiation and expression of mature neural markers in these cells Conclusion: Our results propose that hASCs-exosomes may facilitate the way for the non-cellular treatment of neurodegen-erative diseases.

کلیدواژه ها:

Exosome ، Adipose Stem Cell ، Proliferation ، Differentiation