Utilizing miRNA-143 and miRNA -206 Capsulated in PLGA on Induction of Apoptosis in EL4 Cancerous Cells

Background: One in 250 cases of childhood cancer reaches adulthood, Increasing the likelihood of survival until adoles-cence creates the need for reproduction. According to statistics, more than 20-30% of these children have oligospermia in pu-berty. More than two-thirds of the parents of cancerous children want to freeze the testicular biopsy in the hope of a child s fu-ture fertility. This method has so far been tested in animals and has had successful results. But the major concern in this way is the possibility of biopsy tissue contamination with non-tumor cells, Today, many micro RNAs are used to induce apoptosis in cancer cells. In this study, the effect of transfection of micro RNAs 143 and 206 on apoptosis induction in cancer cells was investigated.Materials and Methods: EL4 Cancer cells were purchased from the Pasteur Institute, in order to encapsulate micro-RNA in the PLGA polymer structure, emulsion-solvent penetration was used. Surface functionalization of micro RNAs -loaded PLGA NPs is done with folate to better targeting. In order to obtain optimal dose from each micro, several doses based on previous studies were evaluated in the dosimetry stage. So that cells {EL4} were treated separately at 3 doses {for micro RNA 143 doses of 25,50 and 75 ng and for micro RNA 206 doses of 50,100 and 150 ng}.Results: The experiments were performed three times. Based on MTT the best dose of mir 143 was 75 ng EL4 viability (45%), and for mir 206 was 150 ng EL4 viability (28%), and when both of them was used EL4 viability was 37%.Conclusion: PLGA-Capsulated microRNA is a new method for apoptotic induction in cancer cell to achieve the pure cell after biopsy and healthy transplantation.

کلیدواژه ها:

Nanoparticle of PLGA ، Micro RNA ، Apoptosis