Effect of Human Umbilical Cord Perivascular Cells on Improving Functional Recovery Following Cerebral Is-chemia/Reperfusion Injury

Background: Ischemic stroke occurs due to obstruction of one of the main arteries of the brain. This situation can lead to neuronal death. Tissue plasminogen activator (TPA) is only accepted treatment for acute ischemic stroke. Mesenchymal stromal cells (MSCs) display therapeutic successfulness. The human umbilical cord perivascular cells (HUCPVCs) have the potential for brain regeneration and protection after injury. The purpose of this study was to investigate the effect of HUCPVCs on functional recovery and neuroprotection.Materials and Methods: Ischemia was induced by middle cer-ebral artery occlusion (MCAO) of male wistar rats. Animals classified to Sham, MCAO, MCAO+HUCPVCs groups. Treat-ment carried out 2 hours after ischemia. Adhesive removal test carried out for evaluation of functional recovery. The mRNA and protein expression of MAP2 were assessed in the cerebral cortex. Nissl staining performed on histological sections to evaluating the number of dead neurons in the ischemic bound-ary zone (IBZ).Results: Our results demonstrate that HUCPVCs adminis-tration noticeably improved functional recovery following MCAO. The number of dark neurons decreased significantly in HUCPVCs therapies group compared to the MCAO group 7th day post-ischemia (p <0.05). HUCPVCs enhanced the neuronal density of MAP2 in the IBZ.Conclusion: In conclusion, our study revealed that the HUCPVCs improved sensorimotor function possibly through reduction of dead neurons and increasing density of mature neurons after cerebral ischemia.

کلیدواژه ها:

Ischemia ، HUCPVCs ، MCAO ، MAP2 ، Dark Neurons