Enhanced Osteogenic Differentiation of Mesenchy-mal Stem Cells Using Electromagnetic Field and Platelet Rich Plasma Modified Scaffolds

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 313

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شناسه ملی سند علمی:

RROYAN20_081

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Recent developments in bone tissue engineering have heightened the need for development of safer and more rapid techniques along with cost-effective strategies. Utiliza-tion of autologous products has become additionally desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tis-sue engineering owing to its huge number of growth factors in-volved in osteogenesis and bone regeneration. Electromagnetic field (EMF) has proven to have inductive effects on some deter-mination pathways of stem cells. Based on studies, biophysical and biochemicals stimuli can facilitate cell differentiation. In the present study, the effect of EMF, as a biophysical factor, and PRP, as a biochemical factor, alone and in combination with each other on osteogenic differentiation has been investigated. Materials and Methods: To accomplish this, we coated Poly-ethersulfone/ Polyvinyl alcohol (PES/PVA) nanofibrous scaf-folds with PRP and, cultured adipose derived stem cells (AD-SCs) on the scaffolds with electromagnetic field exposure, for 14 days. Then, common osteogenic markers were assayed by Real Time PCR. Alkaline phosphate (ALP) activity, calcium mineral deposition and Alizarin red staining were performed. Results: The results revealed that the best osteogenic differ-entiation occurred when cells were cultured on PRP coated PES/PVA scaffold. The exposure of electromagnetic field and presence of osteogenic factors did not show synergistic or addi-tive effects. Among various combinations of already approved osteo-inductive factors, namely PRP, EMF and exogenic osteo-genic factors, the best result was achieved by the simplest and least cost strategy, i.e. merely by PRP coating of the surface.Conclusion: PRP modified PES/PVA scaffolds by itself can maximally induce osteogenesis without the need for any exter-nal physical and/or biochemical stimulations. The major con-tribution of this paper to the current research on bone regen-eration is to establishing the effects of PRP-coated scaffold on osteogenesis.

کلیدواژه ها:

Bone Tissue Engineering ، Adipose Derived Mesen-chymal Stem Cell ، Electromagnetic Field ، PRP ، Nanofibrous Scaffold

نویسندگان

M Kazem Arki

Department of Biology, Islamic Azad university- Science and Re-search Branch, Tehran, Iran

MS Kashef-Saberi

Department of Biology, Islamic Azad university- Science and Re-search Branch, Tehran, Iran

H Hanaee-Ahvaz

Department of Tissue Engineering, Stem Cell Technology Re-search Center, Tehran, Iran

M Kabiri

Department of Tissue Engineering, Tehran University, Tehran, Iran