Cytotoxic and Antioxidant Activity of N-Hexane Fraction of Bandotan Herb (Ageratum Conyzoides L.) and Doxorubicin as Co-Chemotherapeutic Agent in Breast Can-cer Cells ine by In Vitro and in Silico Assay

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 395

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شناسه ملی سند علمی:

RROYAN20_069

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Breast cancer is one of the most common can-cers that causes the highest morbidity and mortality in humans. Some anticancer medicines are not efficient and also have many side effects. Based on the previous research showed bandotan herb extract contained several compounds which potentially have anti-microbial, anti-inflammatory and anti-cancer effects. This study aimed to analyze the cytotoxic activity and co-chemotherapy effect of n-hexane fraction of Bandotan (NFB) combined with doxorubicin in MCF-7 breast cancer cells line by in vitro assay and study the molecular mechanism of the ac-tive component NFB by in silico assay, with HER-2 as protein target.Materials and Methods: Antioxidant activity test of NFB measured by DPPH method and cytotoxicity assay of NFB per-formed with MTT assay method was carried out by molecular docking method between ageratochromene dimer (compound marker of bandotan) on HER-2 protein. NFB also tested by flowcytometer to know the effect of the extract in cell cycle inhibition.Results: Based on the research showed that 1 kg of Agera-tum conyzoides L. obtained 1.5 gram NFB concentrated exc-tract which would then be utilized in antioxidant and cytotoxic test. The docking molecular method results showed that NFB has potential cytotoxic agent mediated by the ability of agera-tochromene dimer inhibited HER-2 protein targets with score affinity of -6.2 kcal/mol and -8.5 kcal/mol with doxorubicin as comparison with affinity of -6.9 kcal/mol on HER-2. Anti-oxidant activity of NFB showed the IC50 value was 493 µg/ ml. The NFB had potency as citotoxic activity with IC50 value of NFB 306 µg/ml to inhibit the growth of MCF-7 cells line and showed synergistic result in combination with doxorubicin as chemotherapy with Combination Index (CI) being between 0.53-0.90. Based on flowcytometry analysis showed that NFB could inhibit the S phase of the MCF-7 cells line.Conclusion: From the result obtained it could be concluded that the NFB has weak potential as antioxidant activity and syn-ergistic effect as co-chemotherapy with doxorubicin that inhibitin S phase in MCF-7 breast cancer cells line based on in vitro and in silico assay.

نویسندگان

R Febriansah

School of Pharmacy, Faculty of Medicine and Health Sciences, Uni- versity of Muhammadiyah, Yogyakarta, Indonesia

WA Lubis

School of Pharmacy, Faculty of Medicine and Health Sciences, Uni- versity of Muhammadiyah, Yogyakarta, Indonesia