Brittle Star’s Arm Regeneration: A Preliminary Gas Chromatography–Mass Spectrometry Study of Molecules Playing Roles in whole Tissue Reconstruction

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 434

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شناسه ملی سند علمی:

RROYAN20_052

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Brittle stars is one of the marine invertebrates has the ability of whole arm reconstruction after arm amputation. However, based on our knowledge there is no information re-garding bioactive chemical molecules have roles in brittle stars’ tissue regeneration. The aim of the present study was to identify molecules have roles in tissue regeneration in brittle star using gas chromatography–mass spectrometry (GC/MS) during the first week of amputation.Materials and Methods: Specimens of adult brittle stars rang-ing from 15-20 cm in overall diameter, were collected and maintained in aquaria containing aerated saltwater at 25°C with a timer-controlled light cycle with 14 hour light and 10 hours dark. Brittle stars were randomly allocated into four groups: in-tact group and 3 hours, 3 days, and 7 days after arm cut groups. In the arm amputated groups, two third of one arm-tip was cut from each brittle star and they were kept in aquarium three hours, three days, and seven days before chopping. Forty grams of whole body of intact and arm amputated brittle stars were chopped and extracted by 200 ml of 70% ethanol solution for 5 days. The hydro-alcoholic extracts were analyzed by GC/MS. Results: Twenty-four compounds detected in hydro-alcoholic extract of intact brittle stars. Three hours after cutting arm, three new compounds were appeared in the analysis compared to the compounds of intact brittle stars. They were suspected to have antiseptic properties such as colchicine, N-desacetyl-N-[4-ace-toxy-3,5-dimethoxycinnamoyl]. Furthermore, three and seven days after cutting the arms, the hydro-alcoholic extracts had 11 different compounds compared to the extract of the three hours after cutting the arms. Four compounds were fatty acids such as linoleic acid ethyl ester, docosahexaenoic acid, 1,2,3propan-etriyl ester, and 1-dodecanol,3,7,11-trimetyl, cholestan-3-one, cyclic 1,2-ethanediyl acetal, (5alpha) have anti-proliferation properties and can induce apoptosis. The other detected com-pounds had unknown biologic effects.Conclusion: GC/MS analysis of hydro-alcoholic extract of brittle stars during the first week of arm regeneration showed that brittle star produces antiseptic and pro-apoptotic molecules which may help it to remove microbial infections and remove excess cells in the healing arm to make a basement for new arm regeneration.

نویسندگان

N Basouli

The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

MR Keshtkar

The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

GHR Mohebbi

The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

AR Barmak

The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran