Endogenous Wnt/ β catenin Signaling Pathway Is Inhibited During Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 278

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شناسه ملی سند علمی:

RROYAN20_049

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Bone repair is one of the most important and ur-gent needs for the society to overcome the problems concerning the skeletal diseases. Nowadays, cell therapy is one of the hope-ful methods. Studies have shown that adipose-derived mesen-chymal stem cells (ADMSCs) can be induced towards the oste-ogenic differentiation both in vitro and in vivo. Among different signaling pathways involved during this process, Wnt/β-catenin plays a significant role at several stages of osteogenesis. There are contradictions however, some of which reveal the osteo-induction but others point at the osteo-inhibition. Our previous results favored the inhibitory effect of the exogenously acti-vated Wnt pathway during osteogenesis. This prompted us to examine how the endogenous Wnt/β-catenin pathway is being altered during osteogenesis of ADMSCs.Materials and Methods: ADSCs were isolated from a 36 year old woman and cultured in high glucose DMEM supplemented with 10% FBS, until 80% confluency. They were then treated with dexamethasone, ascorbic acid and β-glycerol phosphate as the osteogenic differentiation medium. Alizarin red staining and calcium content were evaluated on days 7, 14 and 21 post treatment. To analyse the activity of endogenous Wnt/β-catenin signaling pathway during the osteogenic differentiation, real time PCR was performed for: 1) the Wnt related genes such as cyclin D1 and Wnt3a; 2) DKK1, as the Wnt antagonist; and 3) the osteopontin as the latent osteogenic gene.Results: Our results showed that the amounts of extracellu-lar matrix calcification and calcium deposition were gradually increased during the 21 days of treatment with the osteogenic medium. Coincidentally, the expressions of DKK1 and the os-teopontin were up regulated whereas those of the Wnt3a and cyclin D1 decreased significantly.Conclusion: Our results suggest that ADSCs has the potential to differentiate into the osteogenic lineage during which the en-dogenous Wnt/β-catenin pathway is inhibited especially at the later stages of the osteogenesis.

کلیدواژه ها:

Wnt/β-catenin ، Adipose Derived Stem Cell ، Osteo-genic Differentiation

نویسندگان

F Baghernezhad Shayan

Labaratory of Developmental Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

A Parvaneh Tafreshi

Department of Medical Biotechnology, Institute of Genetic Engi-neering and Biotechnology, Tehran, Iran

B Zeynali

Labaratory of Developmental Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran