Single-Cell Transcriptome of Dugesia Japonica

Background: The planaria Dugesia japonica as an in vivo mod-el for stem cell and regenerative biology is a flatworm contains a large population of pluripotent stem cells -called neoblasts - which enable the remarkable regeneration capacity character-istic of the planarian.Materials and Methods: For the first time, we have profiled thousands of single cells in Dugesia japonica using 10x Genom-ics Chromium Single Cell 3 Solution in order to characterize the different cell populations of the adult worms and under-stand the gene expression changes responsible for neoblast that maintain the homeostasis by continuously differentiating to all adult cell types. Therefore, single cell transcriptome profiling of adults should reveal mature and progenitor cells.Results: Computational analysis of single-cell transcriptomes has allowed us to identify various Head abundant specific cell types, neoblast subpopulations plus major differentiated cell types and subpopulations. Our in silico prediction of future cell states from the transcriptional changes and lineage reconstruc-tion method placed all available major cell types onto single lineage trees that connects all cells to specific cell groups and provides insights into single-cell trajectories. We further eluci-date the contributions of each cell type in the whole organism/ specific tissues using cell type-specific gene expression refer-ences of our scRNA-Seq toward deconvolving of available bulk RNA-Seq.Conclusion: Taken together, our results have extensively char-acterized the different cell types in adult planaria, Dugesia japonica, and demonstrate the importance of single-cell tran-scriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology.

کلیدواژه ها:

Single-Cell Transcriptome ، Dugesia Japonica ، Pla-naria ، Stem Cell and Regenerative Biology ، 10X Chromium