microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 272

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شناسه ملی سند علمی:

JR_IJBMS-22-5_012

تاریخ نمایه سازی: 20 مهر 1398

چکیده مقاله:

Objective(s): microRNA-29 (miR-29) family miRNAs have been mentioned as tumor suppressive genes in several human cancers. The purpose of this study was to investigate the function of miR-29a in nasopharyngeal carcinoma (NPC) cells. Materials and Methods: Human NPC cell line 5-8F was transfected with mimic, inhibitor or scrambled controls specific for miR-29a. Subsequently, cell viability, migration, apoptosis and expression changes of VEGF were assessed by trypan blue staining, MTT assay, transwell assay, flow cytometry, Western blot and RT-qPCR. TargetScan online database was used to predict the targets of miR-29a, and luciferase reporter assay was carried out for testing the targeting relationship between VEGF and miR-29a. Western blot analysis was performed to determine the expression changes of core proteins in PI3K/AKT and JAK/STAT pathways. Results: Overexpression of miR-29a suppressed 5-8F cells viability and relative migration, but increased apoptotic cell rate. Consistently, Bcl-2 was downregulated, Bax was upregulated, and caspase-3 and -9 were cleaved by miR-29a overexpression. VEGF was a target gene of miR-29a. Besides, VEGF silence exerted similar effects like miR-29a, as the viability and migration were repressed and apoptosis was induced. Finally, we found that PI3K/AKT and JAK/STAT pathways were deactivated by miR-29a or VEGF silence. Conclusion: These findings highlighted the tumor suppressive effects of miR-29a on NPC cells, as its overexpression inhibited 5-8F cells viability, migration, and induced apoptosis. miR-29a exerted tumor suppressive functions might be via targeting VEGF and deactivating PI3K/AKT and JAK/STAT pathways.

کلیدواژه ها:

5-8F cell ، miR-29a ، Nasopharyngeal carcinoma- (NPC) ، PI3K/AKT and JAK/STAT- pathways ، VEGF

نویسندگان

Qingyuan Shi

Department of Otorhinolaryngology, HwaMei Hospital, University of Chinese Academy of Sciences (Ningbo No.۲ Hospital), Ningbo, Zhejiang ۳۱۵۰۱۰, P.R.China

Jinhua Dai

Department of Clinical Laboratory, HwaMei Hospital, University of Chinese Academy of Sciences (Ningbo No.۲ Hospital), Ningbo, Zhejiang ۳۱۵۰۱۰, P.R.China

Lizhen Huang

Department of Otorhinolaryngology, HwaMei Hospital, University of Chinese Academy of Sciences (Ningbo No.۲ Hospital), Ningbo, Zhejiang ۳۱۵۰۱۰, P.R.China

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