Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchestan, Iran
محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 5، شماره: 1
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 411
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شناسه ملی سند علمی:
JR_AJP-5-1_001
تاریخ نمایه سازی: 1 مهر 1398
چکیده مقاله:
Objective: Boerhaavia elegans L. (Nyctaginaceae) is a medicinal plant used for the treatment of kidney disorders, urinary tract disorders and blood purification in Baluch tribe. The aim of present study is to evaluate the antioxidant propertyof B. elegans species for the first time. Materials and Methods: Different parts (leaf, stem and fruit) of the plant were extracted by using various solvents (water, methanol, chloroform and ethyl acetate) and evaluated for their antioxidant activity using DPPH (2, 2-diphenyl-1 picryl hydrazyl) and FRAP (ferric reducing antioxidant power) methods. In addition, total phenolic content was determined by Folin–Ciocalteu reagent. Results: Antioxidant results were expressed as IC50. The antioxidant power in DPPH and FRAP assay were evaluated as shown in decreasing order: Methanolic extract > Aqueous extract > Ethyl acetate extract > Chloroform extract, for all parts of the plant. In both methods of antioxidant assay and Folin-Ciocalteu method, methanolic extract of leaf exhibited the highest activity and the most phenolic content IC50= 6.85ppm and 16.41 mg GA/g d w respectively. Total phenolic content had a positive relationship with antioxidant capacity in extracts and there was a high correlation (r=1.00, p<0.01) between antioxidant activities as determined by both antioxidant assays for various parts. Conclusion: The results of the experiments showed that B. elegans extract had significant antioxidant effects. This high antioxidant activity may be linked to phenolic contents of the plant but complementary investigations are suggested in order to determine active elements.
کلیدواژه ها:
نویسندگان
Zahra Sadeghi
Department of Production and Utilization of Medicinal Plants, Faculty of Agricultural and Natural Resources, Higher Educational Complex of Saravan, Saravan, I. R. Iran
Jafar Valizadeh
Department of Biology, University of Sistan & Baluchestan, Zahedan, I. R. Iran
Omid Azyzian Shermeh
Department of Biology, University of Sistan & Baluchestan, Zahedan, I. R. Iran
Maryam Akaberi
Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
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