Preparation of Functionalized Graphene Oxide Loaded with Silver Nanoparticles and Investigation of its Antibacterial Activities

Metal nanoparticles are a particular class of nanomaterials that have garnered huge interest inthe field of scientific research, and in many other technical disciplines, due to their chemicaland physical properties and the numerous effective applications [1]. Silver nanoparticles hasbeen proved to be the most effective antimicrobial agent. Ag NPs has antibacterial activityagainst gram negative and gram positive bacteria. Some of the most problematic multi drugresistant (MDR) microorganisms that are encountered currently include Pseudomonas aeruginosaand Staphylococcus aureus [2]. In this article, the antibacterial activity of nanocatalystwas evaluated by agar well diffusion method against to two bacteria, Staphylococcus aureus(ATCC29737) as Gram-positive and also Escherichia coli (ATCC25922) as Gram-negative bacteria.Firstly, GO and SOCl2 were added to generate GO-Cl and then melamine was added toGO-Cl under nitrogen atmosphere. The obtained product was treated with methyl methacrylateand ethylenediamine, then AgNO3 was added into the catalyst and nanocatalyst was preparedby plant extract. The result has been shown that these nanocomposites have good effecton both gram negative and gram positive bacteria. The nanocatalysts have been shown moreantibacterial activity against Staphylococcusa aureus bacteria (Fig. 1).