Influences of mesenchymal stem cells differentiate to bone, cartilage and fat cells after knocking down of Toll-like receptors

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 332

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شناسه ملی سند علمی:

RMED08_235

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Mesenchymal stem cells (MSCs) are multipotential stem cells that can differentiate into osteoblasts, chondrocytes and adipocytes. MSCs thought to be excellent candidate tools for the field of regenerative medicine, due to their differentiation potential. Therefore, it is important to reveal the molecular mechanisms that regulate the MSC function including survival, proliferation and differentiation. TLRs, which expressed in MSCs, are critical in regulating the fate decision of MSCs. In addition to pathogen-associated molecular patterns, a variety of endogenous agonists recognized by TLRs.Objective: In this study, we investigated the role of TLRs activation (both endogenous and exogenous) on differentiation potential of mesenchymal stem cells into three lineages. We also aimed to investigate the effect of TLRs knockdown on the differentiation of human mesenchymal stem cells (MSCs) from bone marrow.Materials and Methods: MSCs were stimulated with endogenous and exogenous ligands. TLR3 and TLR4 expression was knocked down by psiRNA. The differentiation potential of MSCs was evaluated in adipogenic, chondrogenic and osteogenic differentiation media by gene expression related to the differentiation at day 7, 14, and 21. TLRs mRNA expression assessed by Real Time PCR in the treated and control groups.Results: The regulatory role of TLRs was identified on mesenchymal stem cell differentiation and was found that activated TLRs by their ligands promote the osteogenic and adipogenic differentiation of MSCs in vitro.Conclusion: These results suggested that TLRs regulates bone marrow MSC adipogenic and osteogenic differentiation. These data also provide new insight in developing new therapy in bone regeneration using MSCs by toll-like receptors ligands.

کلیدواژه ها:

TLRs ، Knockdown ، Human mesenchymal stem cells ، Differentiation ، Gene expression ، Ligands

نویسندگان

F Khodabandehloo

Department of Molecular Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

M Nassiri-Asl

Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

F Rajaei

Fertility and Infertility Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran

Z Zandieh

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran