Targeted cytotoxicity of bilayer nanogel containing oxaliplatin on colon cancer cell line (HT29) and analysis of BAX and Bcl2 gene expression

oxaliplatin is the newest platinum derivative in chemotherapy with promising activity in colorectal cancer but side effects, limited cellular uptake and death is still a problem. In this paper, we report a bilayer Hyaluronic acid-Alginate nanogel (Ha-Al NG) that indicate to be appropriate in the delivery of oxaliplatin as model drug loades into the Ha-Al nanogels . The prepared samples were characterized by techniques like FTIR and SEM. .The results revealed that the Ha-Al nanogels are cytocompatible. . These nanogels can rapidly be taken up by HT-29 cells (a colon cell line). .. The highest cytotoxicity for Ha-Al NG was after 72 h with IC50=4.7 and this was after 72h with IC50=7.09 for oxaliplatin. Compared to pure oxaliplatin and Al NGs, the Ha-Al NGs has lower IC50 value and superior cytotoxicity in HT29 cells and it can indicate the effect of drug targeting that leads to more induced apoptosis and less side effects. there was no significant cytotoxicity observed in Ha-Al NGs and Al NGs toward HT29 cells.. In addition, a higher oxaliplatin intracellular accumulation and a significant cell death extension by apoptosis mechanism is notice when compare with free oxaliplatin . For apoptosis analyse the mRNA levels of Bax and Bcl-2 genes were analyzed by Realtime PCR. Accordingly, the developed nanogels can be employe as an excellent candidate to overcome the inefficiency of oxaliplatin in anticancer treatments.

کلیدواژه ها:

oxaliplatin ، apoptosis ، bilayer Hyaluronic acid-Alginate nanogel ، nanoparticles