Evaluation of Cytotoxic Effect of Betatrophin on HepG2 Cell-Line by MTT ASSAY

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 376

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شناسه ملی سند علمی:

CANCERMED04_011

تاریخ نمایه سازی: 17 مرداد 1398

چکیده مقاله:

Introduction & Aim: Hepato Cellular Carcinoma (HCC) represents a major public health problem with a high impact on society and is the third reason of cancer-related deaths worldwide. Because of diagnosis at late stages it has poor prognosis and molecular mechanisms of this cancer has also remained largely unknown. Betatrophin is a novel detected protein which has high level expression in HCC. In this study we examined the effect of this protein on HepG2 cell line. Methods: Recombinant betatrophin was expressed in BL21 and purified by Ni-NTA chromatography. MTT assay was conducted on human liver carcinoma cells (HepG2) to evaluate the cytotoxic effect of recombinant betatrophin on them. Results: It was appeared that betatrophin has low toxicity against HepG2 cancer cells and can reduce cell growth to 30%. Conclusion:It seems that the upregulated expression of betatrophin in hepatocellular carcinoma is more likely to be a response than acting as a tumorgene because otherwise it wouldincrease the proliferation of cells. However additional studies are needed to elucidate the role of betatrophin in HCC.

نویسندگان

Nastaran Monzavi

Department of Cell & Molecular Biology, School of Biology College of Science, University of Tehran, Tehran, Iran

Nematollah Gheibi

Department of Biothechnoligy, Faculty of Paramedicals Qazvin University of Medical Sciences, Qazvin, Iran

Seyed Jalal Zargar

Department of Cell & Molecular Biology, School of Biology College of Science, University of Tehran, Tehran, Iran