Synthesis of graphene-based conductive composites as a nanostructured cardiac patch for potential application in heart regeneration

IntroductionNowadays, myocardial infarction serves as the main cardiovascular system diseases. Considering the novel approaches of regenerative medicine, tissue engineering-based products seem promising for accelerated treatment of heart failure. Refereeing to the role of cardiac electrophysiology in the regeneration of the damaged heart failure, conductive heart patches are under development.ObjectivesThe purpose of this study is synthesis and characterization and optimization of nanostructured electrospun polyethylene terephthalate (PET)/graphene oxide (GO) conductive mats as a cardiacpatch.MethodsGO nano-particles were synthesized by modified Hummer method and dispersed at different concentrations in PET solution (TFA/DCM) at a 1: 1 ratio. Two different nanostructure of the nano-fibrous PET/GO mats were prepared by using blending and core-shell electrospinning method. chemical, mechanical, physical and biological properties of the prepared nanocomposites were comprehensively evaluated to select an optimized formulation.Resultsbased on optical and fluorescent microscopy results, the uniformed bead-free morphology at 40 ug/ml GO concentration by The physical properties such as conductivity and hydrophilicity and some mechanical properties were studied and all of them were improved also morphological properties were investigated and fibers diameters were changed in two series of composite and core-shell samples. Results of biocompatibility and cell adhesion studies by HUVEC cells showed significant cell proliferation and interactions with optimized nanocomposite formulation compared to bare PET nanofiber. This study accepts the beneficial effect of GO nanoparticle in increasing electroactivity of PET mats as well as improving the cell responses .ConclusionThe results showed that both blended and core-shell mats containing 40 ug/ml GO concentration, could be candidates as an appropriate substrate for regeneration heart damage in the case of conductive cardiac patch

کلیدواژه ها:

Nanocomposite ، Cardiac patch ، PET ، Graphene oxide ، Electro conductivity