Delivery of growth factors using a smart polymer for angiogenesis in osteoinductive scaffold

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 357

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شناسه ملی سند علمی:

ITERMED01_353

تاریخ نمایه سازی: 7 مرداد 1398

چکیده مقاله:

Introduction Bone tissue engineering (BTE) faces a major challenge about the cell viability after implantation of a construct. The lack of vascularization within the implant is a key challenge in BTE. Endothelial differentiation within a bone scaffold can be an approach to improve the vascularization. MethodsIn this study, temperature-sensitive Poly (N-isopropylacrylamide) (PNIPAM) nanoparticles was first synthesized by free radical polymerization technique. Vascular endothelial growth factor (VEGF) loaded PNIPAM nanoparticles (VEGF-PNIPAM) was then prepared by diffusion methods. The 3D PLGA/β-TCPscaffolds were also synthesized byparticle leaching technique. Human Bone Marrow Mesenchymal Stem Cells (hBMSCs) were seeded on the scaffolds and VEGF-PNIPAM nanoparticles were injected into the cell-seeded scaffolds in osteogenic culture medium. The scaffold without VEGF-PNIPAM nanoparticles was used as the control group. The effects of released VEGF on the differentiation of hBMSCs into endothelial cells in osteogenic culture medium were investigated using Scanning Electron Microscope (SEM) and Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR) techniques after 4, 7 and 14 days. ResultsThe results showed that the PNIPAM nanoparticles had spherical grains in the range of 50–150 nm. The cumulative percentageVEGF release after 72 h was 70%. Morphology of the scaffolds with and without VEGF-PNIPAM nanoparticles studied by scanning electron microscope showed that in the presence of VEGF-PNIPAM nanoparticles proliferation, cell viability and cell adhesion were increased compared to control group. QRT-PCR also revealed expression of CD31, vWF and KDR as angiogenic markers compared to control group. The study of released VEGF effects on hBMSCs by QRT-PCR and morphological investigation confirmed the endothelial differentiation in osteogenic culture medium after 7 and 14 days. The obtained data showed that slow delivery of the VEGF along with osteogenic stimuli enhanced both osteogenic and the angiogenic differentiation of hBMSCs. ConclusionAccording to the given data the growth factors (GFs) loaded PNIPAM nanoparticles is promising nanosystem that can be applied to deliver the bioactive GFs for tissue engineering applications. Our data showedthe VEGF-PNIPAM nanoparticles have the potential to load and release the angiogenic GFs for stimulation of angiogenesis in bone tissue engineering

کلیدواژه ها:

Smart poly (N-isopropylacrylamide) nanoparticles ، Angiogenesis ، 3D scaffold ، Bone tissue engineering

نویسندگان

Afsaneh Adibfar

Department Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Iran

Ghassem Amoabediny

Faculty of Chemical Engineering, University of Tehran, Iran

Mohamadreza Baghaban Eslaminejad

Department of Stem Cells and Developmental Biology, Royan Institute, Iran

Javad Akbari

Faculty of Chemical Engineering, University of Tehran, Iran