Study of adult stem cellseffects on ovine cytokines following acute respiratory distress syndrome (ARDS)

Introduction ARDS is the most commonclinical syndrome. The successful treatments for lung diseases are very limited and drug therapies in many cases inefficient. Most lung diseases do not respond to existing treatments, so finding better treatment for ARDS is essential. Objectives In this study, we investigated the therapeutic effect of BM-MSC in the control of lung inflammation.Methods 10male adult sheep was used into two groups. An experimental model of ARDS was induced by endotracheal endotoxin of E. coli strains-O55: B5 infusion and confirmed. In the treatment group, stem cell from bone marrow was isolated, andafter 24 hours of ARDS-induce, 5×107 cells/sheepwere engrafted. For evaluate effects of cell therapy, BAL sample was collected, and cytokine includes IL10, IL6 and TNFα were measured by ELISA kit before and 12,24,48,72 and 168 hours after MSCs transplantation or PBS infusion.Results The BAL findings indicated that IL6 and TNFα levels were significantly increased in both groups in response to LPS. After cell therapy, pro-inflammatory cytokine levels were reduced. IL6 was a significant difference at24,48,72 and 168h compared with baseline and treatment group compared with the control group were significantly at times of 24,48,72 and 168h. TNFα was also found at24,48,72h in treated groups were significantly lower than baseline and the treatment group compared with the control group were significantly at times 24,48 and 168h(P<0.05). Increase levels of IL-10 correlated significantly in the treatment group at hours of 24, 48,168 and compared with a control group at times of 24 and 48h.Conclusion The results showed that local administration of BM-MSCs could regulate inflammatory response and significantly reduces ARDS complications in sheep

کلیدواژه ها:

BM-MSCs ، ARDS ، BAL ، Sheep