CT-scan an approach for the diagnosis and follow up stem cell therapy in respiratory failure

IntroductionAcute respiratory distress syndrome (ARDS) is one of the main cause and developing factor of respiratory failure. A specificfeature of ARDS is a loss of aerated lung volume. Chest computerized tomography (CT-scan) is a non-invasive and repeatable techniquefor diagnosis of lung inflammation and edema. CT-scan can measure lung densities and separated aerated from poorly and nonaerated lung regions. Objectives Our aim is a study of CT-scan roles on stem cells therapyeffects in repair and regeneration ARDS in sheep. Methods An experimental model of ARDS was made in 10 male, white New Zealand rabbits (n=5 rabbits in each experimental and control group) by intratracheal deliveryE.Coli-lipopolysaccharide and inflammation confirmed by imaging. Then, in theexperimentalgroup,BM-MSCs were harvested and expanded, and 107 cells were intratracheally autographed. Conventional CT from lung was obtained at 12h and 1, 2, 3 and 7days after transplantation. Hounsfield unit and volumes of aerated and non-aerated parts of the right and left lung were measurements using a computerized tomographic. Results The result demonstrated the Hounsfield unit had increased one day after the inflammation that represents replacement of alveolar air with mucus and inflammatory cells.The stem cell therapy caused a significant decrease in Hounsfield unit at 48, 72 and 168h post-transplantation compared with inflammation time which is indicating an increase in air volume of the lung in the treatment group. Also, the mean lung volume in both groups increased after inflammation, but transplantation of BM-MSCs had decreased in the process at 72and 168h. Conclusion CT has a key role in the diagnosis and management of ARDS and is a tool to identify complications

کلیدواژه ها:

ARDS ، BM-MSCs ، CT-Scan ، Lung ، Rabbit