Alginate sulfate immobilization on PCL nanofibers by cold atmospheric plasma induces bone marrow mesenchymal stem cells proliferation

IntroductionNowadays, cartilage tissue engineering is used as a potential solution for replacing or repairing damaged tissues or organs. In this field, the suitable context for growth of the cell is provided in natural space by using electrospun nanofibers and by providing space such as the 3D cell space.Objectives This study was carried out aimed to investigate mesenchymal stem cells behavior on a PCL nano-fiber substratemodified with gelatin and sulfate alginate by cold atmospheric plasma (CAP).Methods PCL nanofibers were prepared using electrospinning method. After modification of the surface with CAP, it was grafted with different percentages of gelatin and alginate sulfate, then the growth and the proliferation of mesenchymal stem cells on the scaffold was evaluated. Biocompatibility and proliferation of cells were investigated using MTT assay at 24 and 72 hours. An electron microscope and DAPI test were used to confirm the presence of cells on the scaffold.Results According to the results of scanning electron microscopy and optical spectrum measurements, modified nanofiber scaffold, PCL- Alginate sulfate 25 have a better structure. Biocompatibility results show that cells exhibit better viability and adhesion on the scaffold surface.ConclusionAccording to the results of this study, modified PCL scaffolds that have been grafted with 25% of alginate sulfate, compared with other percentages, are suitable for proliferation of mesenchymal stem cells. So, this can be a good candidate for tissue engineering.

کلیدواژه ها:

PCL Nanofibers ، Cartilage tissue engineering ، Cold atmospheric plasma