Investigation of chondrogenesis of human adipose derived stem cells on the poly (ε-caprolactone)/devitalized cartilage matrix/fibrin hybrid scaffolds prepared via sandwich model

IntroductionNative extracellular matrix (ECM) is comprised of a complex network of structural and regulatory proteins that are organized into a fibrous matrix. The multifunctional nature of native ECM will need to be considered and confidently mimicked in the design and fabrication of tissue-engineered scaffolds. The introduction of a natural/polymer composite such as Poly(εcaprolactone) (PCL)/ECM offers both promising mechanical properties and binding sites for cell attachment and proliferation.ObjectivesInvestigation of chondrogenesis of human adipose derived stem cells on the poly (ε-caprolactone)/devitalized cartilage matrix/fibrin hybrid scaffolds prepared via sandwich modelMethodsThe current study established a new sandwich model for cartilage tissue engineering. PCL/ devitalized cartilage matrix (DCM) nano fibrous electrospun by incorporation of DCM particles into PCL mats were fabricated. By seeding fibrin/ human adipose derived stem cells (hADSCs) on the mats and stacking them layer-by-layer, we successfully engineered the cartilage-liked scaffolds in vitro. The PCL/DCM/Fibrin scaffold support the cell growth and promote the formation of the new tissue. PCL and PCL/DCM nano fibrous electrospun were fabricated according to our previous work. To investigate the influence of incorporation of DCM particles into PCL mats on the chondroinductivity of the scaffolds, hADSCs were seeded on the scaffolds by fibrin hydrogel at final seeding density of 2.5×106 cells/ml fibrin via sandwich model.ResultsThe genes expression of sex determining region Y-box9, aggrecan, types II and X collagens (COL) were assessed by real-time polymerase chain reaction. Biochemical assay was performed to evaluate the glycosaminoglycan (GAG) synthesis in the scaffolds. The findings of this study demonstrate that DCM incorporated into electrospun PCL fibers influences GAG synthesis and COL II gene expression by hADSC. In vitro culture showed that the TGF-β3 growth factor could help hADSCs to differentiate into cartilage cells and increase the synthesis of cartilage specific matrixes on PCL/DCM/fibrin scaffoldConclusionPCL/DCM/fibrin scaffold induces chondrogenesis

کلیدواژه ها:

Electrospun ، Cartilage drive matrix ، Sandwich model ، Hybrid scaffolds ، Chondrogenesis