Differentiation and viability of human adipose-derived stem cells into schwann-like cells at the presence of laminin

IntroductionRegeneration of peripheral nerve injuries (PNI) is a complicated process. Schwann cells (SCs) play an important role in nerve regeneration but obtaining SCs is limited, therefore, stem cell transplantation is a promising strategy for PNI. Human Adipose-Derived Stem Cells (hADSCs) are multipotent specific stem cells that are suitable case, also, many factors are critical for nerve repair including growth factors production and extracellular matrix (ECM) molecule. Laminin is an essential protein in ECM synthesize by SCs and necessary for increasing proliferation and regulating the morphology of SCs.ObjectivesThe purpose of this study is to induce ADSCs into SC-likecells and assess the effect of laminin-coated culture on the efficiency of SC-like cells differentiation.MethodsHADSCs are induced into neurospheres and then re-plated in present and absent of laminin flasks with differentiation medium and then MTT assay is run to exam the viability ofSC-like cells after induction.ResultsThe phenotype of the differentiated ADSCs are converted to bi-polar or spindle shape in the absence and presence of laminin. The survival rate of SC-like cells on a laminin matrix is significantly higher than that of the cells grown on a plastic surface (P < 0.05).ConclusionThis phenomenon suggests that the stem cells harvested from adipose tissue in presence of laminin can induce appropriate microenvironment for nerve repair and the potential of being applied in neurodegenerative diseases.

کلیدواژه ها:

Adipose-derived stem cells ، Laminin ، Schwann cell