Fabrication of 3D Scaffolds based on nano-biomimetic collagen hybrid constructs for skin tissue engineering

Three dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frameworks for skin tissue engineering. In this study, hybrid constructs of 3D scaffolds were successfully fabricated by freeze-drying method from combinations of the type I collagen (Col), and synthetic poly (lactic acid) (PLLA) or polycaprolactone (PCL). Four different groups of 3D porous scaffolds including PCL, PCL-Col, PCL-PLLA, PCL-PLLA-Col were fabricated and systematically characterized by HNMR, FT-IR and SEM. Adipose tissue derived mesenchymal stem cells (AT-MSCs) were seeded in all scaffolds. Subsequently, the viability, proliferation and adhesion of the cells were investigated using MTT assay and scanning electron microscopy (SEM). The results showed that scaffolds containing Col, particularly PCL-PLLA-Col scaffold, with pore sizes close to 400nm and sufficiently interconnected, have significantly greater potential (p<0.01) for encouraging AT-MSCs adhesion and growth. The PCL-PLLA provided a mechanically stronger mesh support and the type I Col microsponges encouraged excellent cell adhesion and tissue formation. The scaffold with the best properties could be an appropriate functional candidate for artificial skin constructs

کلیدواژه ها:

Three Dimensional Porous scaffolds ، collagen ، Adipose tissue derived stem cells ، Poly caprolacton-Poly L-Lactic acid ، Tissue engineering