Decellularized Wharton’s jelly as an appropriate material for tissue engineering application

IntroductionThe Wharton’s jelly (WJ) of human umbilical cord is rich of natural extracellular matrix (ECM). This material can be used to facilitate cellular attachment, proliferation, infiltration, and differentiation, also lead to constructive ECM remodeling and tissue reconstruction. ObjectivesIn this study, we produced a new decellularized Wharton’s jelly material (DWJ) as a biomaterial for tissue engineering applications. Methods Wharton’s jelly (WJ) of human umbilical cord was isolated from surrounding tissues and placed in 5% SDS for 6 hours. The tissue was then washed 3 times in PBS and placed in 1% Tritin-X100 for 3 hours. All of these stages were done in 37 0C in shaker incubator. DAPI staining was used for decellularization approval. DWJ was freeze-dried and milled to create a powder. The size of decellularized tissue particles was defined using Dynamic Light Scattering (DLS) analysis. Results DAPI stainingindicated that there are no cells in decellularized tissue. DLS analysis showed that the size of decellularized particles is about 300 micrometer.ConclusionThis powder can be used alone or in combination with other materials to make a scaffold as an appropriate substrate for cell seeding in tissue engineering.

کلیدواژه ها:

Wharton’s jelly ، Decellularization ، Scaffold ، Tissue engineering