Effects of co-administration of bone marrow stromal cells and granulocyte colony stimulating factor on the recovery of damaged ovaries by chemotherapy in rat

IntroductionBone marrow stromal cells (BMSCs) as a type of mesenchymal stem cells (MSCs) besides granulocyte-colony stimulating factor (G-CSF) as a type of growth factors may recover damaged ovaries.ObjectivesThe aim of this study was to investigate the effects of co-administration of BMSCs and G-CSF on injured ovaries after creating chemotherapy model with cyclophosphamide in rat.MethodsBMSCs were cultured and confirmed by CD specific markers of stromal cells. Female rats were injected intraperitoneally with cyclophosphamide for 14 days for inducing chemotherapy and ovarian destruction. Then, BMSC injected into bilateral ovaries and also intraperitoneal injection of G-CSF was performed individually and together. Four weeks later, the rate of ovarian follicles evaluated using H&E staining, the rate of apoptotic granulosa cells assessed using TUNEL assay, and the number of produced oocytes from the ovaries and the levels of serum E2 and FSH detected using ELISA kit.ResultsOur results showed the effect of co-administration of 2×106 BMSCs and 70 μg/kg of G-CSF were significantly more effective than the control group (P<0.001), BMSC group (P=0.016) and G-CSF group (P<0.001) on the recovery of damaged ovaries.ConclusionThe effect of co-administration of BMSCs and G-CSF is probably more effective than the effect of their separate administration on the recovery of damaged ovaries by chemotherapy.

کلیدواژه ها:

Bone marrow stromal cells ، Granulocyte colony stimulating factor ، Chemotherapy ، Ovary ، Regeneration