Wound healing with using of nanofiber PLA scaffold

Introduction Skin is the largest organ in the body that consider as a first barrier against toxins and microorganisms in the environment. Injury of this tissue mainly causes infection in local and distributes to whole the body with blood circulation. Objectives During the last decade, a number of researches done about all type of wound healing. Hence, the present study aimed to use the tissueMethodsIn this study, we used tissue engineering method for treatment of the wound. For this purpose, first, we fabricated and characterized nanofiber polylactic acid (PLA) scaffolds with electrospinning technique. In the next step, bone marrow stem cells isolated from femur bone of rat seeded on the scaffolds. Then, wound models were formed through removal of the full thickness of skin with 2cm round diameter on the back of rats. In this research, we investigated four groups with PLA pure scaffolds, PLA scaffolds with seeded stem cells, Hemofoam (ChitoTech Inc, Iran) as a control dressing and a control group without any dressing. In 7, 14 and 21 days, we monitored the process of wound healing.ResultsBy means of H&E and Masson’s trichrome staining and detection of keratin 10 (Krt10),keratin 14(KRT14) and involucrin ( IVL) genes by RT-PCR, we fund that the PLA scaffold group with bone marrow stem cells indicated best result rather than Hemofoam and pure PLA scaffold during repair of wound, respectively.ConclusionWe suppose that high porosity of this non-woven scaffold that lets cell migration and appropriate transport nutrient elements to cells lead to these good results.

کلیدواژه ها:

Wound ، Healing ، Nanofiber ، PLA ، Scaffold