Effects of pomegranate seed oil on oxidant/antioxidant balance in heart and kidney homogenates and mitochondria of diabetic rats and high glucose-treated H9c2 cell line

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 378

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شناسه ملی سند علمی:

JR_AJP-7-4_004

تاریخ نمایه سازی: 18 تیر 1398

چکیده مقاله:

Objective: Oxidative stress is a major cause of diabetes complications. The present study aimed to investigate the beneficial effects of Pomegranate Seed Oil (PSO) on diabetes-induced changes in oxidant/antioxidant balance of the kidney, heart and mitochondria from rats and H9c2 cell line. Materials and Methods:  In these in vivo and in vitro studies, male rats were divided into four groups (twelve each): group 1 served as control, group 2-4 received a single dose of streptozotocin (60 mg/kg, i. p), groups 3 and 4 received PSO (0.36 and 0.72 mg/kg/daily, gavage), respectively.  After three weeks, six rats of each group and one week later the remaining animals were anaesthetized and the hearts and kidneys were removed and homogenized. Mitochondrial fractions were separated and enzyme activities were measured in each sample. H9c2 cells were pretreated with high levels of glucose (35 mM), and then, incubated with PSO. Finally, cell viability test, reactive oxygen species production and lipid peroxidation were evaluated. Results: Significant reduction in enzymes activity (Superoxide dismutase, Glutathione S-transferase and Paraoxonase 1), compensatory elevation in Glutathione Reductase, Glutathione Peroxidase and Catalase activity followed by reduction after one week and significant elevation in Oxidative Stress Index (OSI) were observed in diabetic group. PSO treatment resulted in a significant increase in enzymes activity and decreased OSI values compared to diabetic group in both tissue and mitochondrial fractions. PSO remarkably decreased glucose-induced toxicity, ROS level and lipid peroxidation in H9c2 cells. Conclusion: Results suggested that PSO has a protective effect against diabetes-induced alterations in oxidant/antioxidant balance in tissues, mitochondrial and H9c2 cell line.

نویسندگان

Hamid Mollazadeh

Department of physiology and pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

Mohammad Taher Boroushaki

Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Soukhtanloo

Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Amir Reza Afshari

Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Aboonabi A, Rahmat A, Othman F. 2014. Antioxidant effect of ...
  • Adams LS, Seeram NP, Aggarwal BB, Takada Y, Sand D, ...
  • Aebi H. 1984. Catalase in vitro. Meth Enzymol, 105: 121-126. ...
  • Afshari AR, Sadeghnia HR, Mollazadeh H. 2016. A Review on ...
  • Baynes JW, Thorpe SR. 1999. Role of oxidative stress in ...
  • Beltowski J, Wójcicka G, Marciniak A. 2002. Species-and substrate-specific stimulation ...
  • Bihamta M, Hosseini A, Ghorbani A, Boroushaki MT. 2017. Protective ...
  • Boroushaki MT, Mollazadeh H, Afshari AR. 2016. Pomegranate seed oil: ...
  • Boroushaki MT, Mollazadeh H, Rajabian A, Dolati K, Hoseini A, ...
  • Boroushaki MT, Rajabian A, Farzadnia M, Hoseini A, Poorlashkari M, ...
  • Bray RC, Cockle SA, Fielden EM, Roberts PB, Rotilio G, ...
  • de Haan JB, Stefanovic N, Nikolic-Paterson D, Scurr LL, Croft ...
  • Dirin MM, Mousavi S, Afshari AR, Tabrizian K, Ashrafi MH. ...
  • Forbes JM, Cooper ME. 2013. Mechanisms of diabetic complications. Physiol ...
  • Fridovich I. 1997. Superoxide anion radical (O· 2), superoxide dismutases, ...
  • Gill SS, Anjum NA, Hasanuzzaman M, Gill R, Trivedi DK, ...
  • Goertz A, Ahmad KA. 2015. Biological Activity of Phytochemical Compounds ...
  • Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferases the ...
  • Ikeda Y, Inoue M, Suehiro T, Arii K, Kumon Y, ...
  • Jain SK. 1989. Hyperglycemia can cause membrane lipid peroxidation and ...
  • Jain SK, Levine SN, Duett J, Hollier B. 1990. Elevated ...
  • Johansen JS, Harris AK, Rychly DJ, Ergul A. 2005. Oxidative ...
  • Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. ...
  • Kang JH. 2003. Modification and inactivation of human Cu, Zn-superoxide ...
  • Khazim K, Gorin Y, Cavaglieri RC, Abboud HE, Fanti P. ...
  • Kowluru RA, Kern TS, Engerman RL. 1997. Abnormalities of retinal ...
  • Madesh M, Balasubramanian K. 1998. Microtiter plate assay for superoxide ...
  • Max SR, Garbus J, Wehman HJ. 1972. Simple procedure for ...
  • Mollazadeh H, Hosseinzadeh H. 2014. The protective effect of Nigella ...
  • Mollazadeh H, Sadeghnia HR, Hoseini A, Farzadnia M, Boroushaki MT. ...
  • Moongkarndi P, Kosem N, Kaslungka S, Luanratana O, Pongpan N, ...
  • Paglia DE, Valentine WN. 1967. Studies on the quantitative and ...
  • Rahman K. 2007. Studies on free radicals, antioxidants, and co-factors. ...
  • Rains JL, Jain SK. 2011. Oxidative stress, insulin signaling, and ...
  • Sampson MJ, Braschi S, Willis G, Astley SB. 2005. Paraoxonase-1 ...
  • Schubert SY, Lansky EP, Neeman I. 1999. Antioxidant and eicosanoid ...
  • Shaban NZ, El-Kersh MA, El-Rashidy FH, Habashy NH. 2013. Protective ...
  • Sheweita S, Mashaly S, Newairy A, Abdou H, Eweda S. ...
  • TA S. 2014. Diagnosis and classification of diabetes mellitus. Diabetes ...
  • Tangvarasittichai S. 2015. Oxidative stress, insulin resistance, dyslipidemia and type ...
  • Tesauro M, Nisticò S, Noce A, Tarantino A, Marrone G, ...
  • Trachtman H, Futterweit S, Bienkowski RS. 1993. Taurine prevents glucose-induced ...
  • Ullah A, Khan A, Khan MI. 2015. Diabetes mellitus and ...
  • Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, ...
  • Vroegrijk IO, van Diepen JA, van den Berg S, Westbroek ...
  • Yuan Y, Jiao X, Lau WB, Wang Y, Christopher TA, ...
  • Zarei M, Fakher S, Tabei SMB, Javanbakht MH, Derakhshanian H, ...
  • نمایش کامل مراجع