Study of antimicrobial effects of several antibiotics and iron oxide nanoparticles on biofilm producing pseudomonas aeruginosa
محل انتشار: مجله علوم نانو، دوره: 4، شماره: 1
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 335
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_NAMJ-4-1_005
تاریخ نمایه سازی: 18 تیر 1398
چکیده مقاله:
Objective(s): Pseudomonas aeruginosa is a nosocomial pathogen resistant to most antimicrobial treatments. Furthermore, it persists in adverse environments thereby forming biofilms on various surfaces. Researchers have therefore focused on antibiofilm strategies using nanoparticles due to their unique physicochemical properties. Superparamagnetic iron oxide nanoparticles (SIONPs) have recently shown to possess antimicrobial and anti-biofilm characteristics. In this study, the effects of SIONPs and some antibiotics were tested against strong biofilmproducing P. aeruginosa isolates.Materials and Methods: 60 isolates of P.aeruginosa were screened for biofilm formation on microtiter plates using 0.1%w/v crystal violet (CV) staining. Twenty isolates producing strong biofilms were selected for further study on the effects of antimicrobial agents. Microdilution method was used to assay twenty isolates susceptible to antibiotics. The effects of antibiotics and SIONPs on biofilm formation were determined by the microdilution method and 0.1% CV staining. The checkerboard dilution technique was used to determine the combined effects of SIONPs and imipenem. Results: In twenty isolates, the rate of resistance to ciprofloxacin, levofloxacin, amikacin, azithromycin was 65, 75, 45 and 95% respectively. SIONPs at 30 µg/ml reduced biofilm biomass in 11 isolates; however it stimulated biofilm formation in 9 isolates. The effects of SIONPs in combination with imipenem in the 10 isolates were different exhibiting synergistic or antagonistic relationships.Conclusion: P. aeruginosa has increasingly developed resistance to many antimicrobial agents but the resistance to nanoparticles is less frequently been reported. However, iron oxide nanoparticles could enhance biofilm production in isolate- dependent manner because they may possibly utilize this nanoparticle as an iron source, an important element in biofilm production. The exact mechanism of these effects however, remains to be elucidated.
کلیدواژه ها:
نویسندگان
Khadijeh Ramezani Ali Akbari
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Ahya Abdi Ali
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :