Curcumin coated gold nanoparticles: synthesis, characterization, cytotoxicity, antioxidant activity and its comparison with citrate coated gold nanoparticles

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 340

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شناسه ملی سند علمی:

JR_NAMJ-4-2_007

تاریخ نمایه سازی: 18 تیر 1398

چکیده مقاله:

Objective(s): Biological applications of gold nanoparticles have limitations because of the toxic chemicals used in their synthesis. Curcumin can be used as reducing as well as capping agent in synthesis of GNPs to eliminate the cytotoxicity. Conjugation of curcumin to gold also helps in increasing its solubility and bioavailability. Materials and Methods: Here we report synthesis of gold nanoparticles coated with citrate and curcumin and of two different sizes via chemical routes. UV-Vis absorbance spectroscopy, Dynamic Light Scattering and Transmission Electron Microscopy were applied to study the average particle size, size stability of the samples and zeta potential. Fourier transform infrared, Raman Spectroscopy and Fluorescence Spectroscopy were applied for detection of curcumin on the surface of GNPs. The antioxidant activity was evaluated using DPPH assay and Cytotoxicity was evaluated by MTT assay.Results: Particles were synthesized of 6 and 16 nm size. The average particle size was found to be 21.7 ± 5.7 by TEM. The zeta potential on the surface of Cur-GNPs was negative and larger than 25 mV which is a sign of their high stability. The stability of these particles (with different coatings but with similar sizes) at different time intervals (up to 3 months) and also in different media like cell culture medium, different buffers, glucose and at different pH conditions have been investigated thoroughly. Appearance of functional groups assigned to curcumin in FTIR and SERS spectra are sign of presence of curcumin in the sample. The quenching of the fluorescence in the presence of GNPs reveals the clear indication of the capping and binding of curcumin with GNPs. Cur-GNP1 (16 nm) were found to exhibit highest antioxidant activity than other gold nanoparticles. Cytotoxicity evaluation using MTT assay on L929 cell line proved curcumin coated gold nanoparticles were non-toxic up to 40 ppm.Conclusion: The results revealed that larger curcumin coated gold nanoparticles were stable and also non-toxic and were found suitable for further in-vitro and in-vivo studies.

نویسندگان

Elnaz Shaabani

Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran

Seyed Mohammad Amini

Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran

Sharmin Kharrazi

Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran

Roksana Tajerian

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran

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  • Mody, V.V., R. Siwale, A. Singh and H.R. Mody, Introduction ...
  • Burke, T.R., B. Ye, X. Yan, S. Wang, Z. Jia, ...
  • Guo, R., Y. Song, G. Wang and R.W. Murray, Does ...
  • Ackerson, C.J., P.D. Jadzinsky, J.Z. Sexton, D.A. Bushnell and R.D. ...
  • Biswal, J., S. Ramnani, S. Shirolikar and S. Sabharwal, Synthesis ...
  • Noruzi, M., D. Zare, K. Khoshnevisan and D. Davoodi, Rapid ...
  • Nath, D. and P. Banerjee, Green nanotechnology–A new hope for ...
  • Mohanpuria, P., N.K. Rana and S.K. Yadav, Biosynthesis of nanoparticles: ...
  • Sathishkumar, M., K. Sneha, S. Won, C.-W. Cho, S. Kim ...
  • Shankar, S.S., A. Rai, B. Ankamwar, A. Singh, A. Ahmad ...
  • Chandran, S.P., M. Chaudhary, R. Pasricha, A. Ahmad and M. ...
  • Ankamwar, B., M. Chaudhary and M. Sastry, Gold nanotriangles biologically ...
  • Sneha, K., M. Sathishkumar, S.Y. Lee, M.A. Bae and Y.-S. ...
  • Vankar, P.S. and D. Bajpai, Preparation of gold nanoparticles from ...
  • Singh, D.K., R. Jagannathan, P. Khandelwal, P.M. Abraham and P. ...
  • Lee, W.-H., C.-Y. Loo, M. Bebawy, F. Luk, R.S. Mason ...
  • Agashe, H., K. Sahoo, P. Lagisetty and V. Awasthi, Cyclodextrin-mediated ...
  • Mohanty, C., M. Das and S.K. Sahoo, Emerging role of ...
  • Mohanty, C., S. Acharya, A.K. Mohanty, F. Dilnawaz and S.K. ...
  • Sindhu, K., R. Indra, A. Rajaram, K. Sreeram and R. ...
  • Sreelakshmi, C., N. Goel, K. Datta, A. Addlagatta, R. Ummanni ...
  • Sindhu, K., A. Rajaram, K. Sreeram and R. Rajaram, Curcumin ...
  • Haiss, W., N.T. Thanh, J. Aveyard and D.G. Fernig, Determination ...
  • Brand-Williams, W., M.-E Cuvelier and C. Berset, Use of a ...
  • Mosmann, T., Rapid colorimetric assay for cellular growth and survival: ...
  • Priyadarsini, K.I., Photophysics, photochemistry and photobiology of curcumin: Studies from ...
  • Wang, Y.-J., M.-H. Pan, A.-L. Cheng, L.-I. Lin, Y.-S. Ho, ...
  • Krishnankutty, K. and P. Venugopalan, Metal chelates of curcuminoids. Synth ...
  • Xue, X., J. Wang, G. Si, C. Wang and S. ...
  • Bich, V.T., N.T. Thuy, N.T. Binh, N.T.M. Huong, P.N.D. Yen ...
  • Mooradian, A., Photoluminescence of metals. Phys Rev Lett. 1969; 22(5): ...
  • نمایش کامل مراجع