Thermodynamic study on the interaction of catechin with Jack bean urease
محل انتشار: چهارمین کنگره ملی مهندسی مکانیک و مهندسی شیمی
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 522
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شناسه ملی سند علمی:
MECECONF04_056
تاریخ نمایه سازی: 8 تیر 1398
چکیده مقاله:
In this work complexation process between catechin and urease is examined using Isothermal Titration Calorimetry(ITC),The,at pH 7at 27°C in tris buffer.The enthalpies of urease +catechin interaction are reported and analysed in term of the Hill equation model.The thermodynamic parameters, enthalpy changes (ΔH) and entropy changes(ΔS) were calculated.These data suggested that hydrophobic interaction was the predominant intermolecular forces stabilizing the complex.,It was found that urease has two positively cooperative binding site for catechin. Ka values show catechin in the second binding sites has higher affinity for binding than the first binding sites.
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نویسندگان
Morteza Vaezi
Department of Chemistry, Imam Khomeini International University, Qazvin, Iran
Adeleh Divsalar
Department of Cell and Molecular Sciences, Kharazmi university, Tehran, Iran
Gholamreza Rezaei Behbehani
Department of Chemistry, Imam Khomeini International University, Qazvin, Iran