The effect of magnetized solvent on rate of oxidation reaction of phenols and aromatic amines compounds by horseradish peroxidase

For the first time, the rate of oxidation reaction of some phenolic derivatives and aromatic amines in the presence of hydrogen peroxide as oxidant in pure and magnetized water,investigated, by enzyme horseradish peroxidase (HRP). Therefore Pyrogallol, Catechol, Resorcinol, Ortho-phenylenediamine, Para-phenylendiamine (PPD), Ortho aminophenol (OAP), Meta-aminophenol (MAP) and Para-aminophenol (PAP) selected for enzymatic oxidation processes. The reaction performed in the presence and absence of magnetized water under identical conditions completely. It was found that the magnetized water decreases enzyme activity due to the change in its structure. The change of enzyme structure and activity were studied through UV-Vis and fluorsence spectroscopy. The dissolved hydrogen peroxide in magnetized water, as enzyme substrate, reduced enzyme activity toward pure water.The results indicate that magnetized water affects on the initial rate of oxidation reaction of selective derivative in through the strength change of hydrogen bonds. The initial rate of Pyrogallol, Resorcinol, OAP, MAP and PAP increased and catechol decreased due to getting weaker intermolecular and stronger intramolecular hydrogen bond [1]. The initial rate of OPD and PPD decreased due to hindrance steric and less exposed to the enzyme’s active site because of enhancement of molecules level and the average number of hydrogen bonds in each molecule in magnetized water [2]. It is worth mentioning that hydrogen radical is removed from the amine group for radical formation and the initiation of reaction in enzymatic oxidation prcesses of aromatic amines [3].