Voltammetry determination of Tramadol by using of glassy carbon electrode modified with Alizarine Red S

A novel and easy method for the determination of Tramadol (TRA) is investigated using a glassy carbon electrode (GCE) modified with thin film of Alizarin Red S (ARS) by electro-polymerization. The electrochemical results showed that ARS/GCE displayed excellent increases in the peak currents and a decrease in over potential of TRA in comparison to bare GCE. The measurements were carried out by using differential pulse voltammetry (DPV), cyclic voltammetry (CV) and chronoamperometry (CA) methods. Application of the DPV method showed that there was a linear relationship between the oxidation peak current and the concentration of TRA over the range of 200-2450 M with detection limit of 1.64 M in phosphate buffer (0.1 M, pH=7). The effects of intromitting substances on the definition of TRA were also investigated and it was found that proposed sensor is very selective. The sensor was applied to the determination of TRA in human plasma serum preparations with satisfactory results [1,2].