synthesis of glucose optical biosensor nanocomposite based on tragacanth gum biopolymer

A hydrogel based on tragacanth gum (TG) biopolymer consisting of synthetic crosslinker has been employed as a biosensing platform via encapsulation of enzyme bioreceptors (e.g., glucose oxidase) and fluorescent reporters (e.g., CdS quantum dot) [1,2]. Enzyme and quantum dot (QD) were physically immobilized within the hydrogel matrix. By using atomic force microscopy and scanning transmission electron microscopy, a three-dimensional network that physically hybridized with QDs and encapsulated enzyme bioreceptors with a minimal leakage has been observed. The prepared hydrogel has been used successfully to the detection of analyte such as glucose by using a photoluminescence quenching of the hybridized QDs