DNA breaking and antibacterial effect of Metoclopramide

Metoclopramide is using for stomach and esophageal problems. It is frequently used to treat and prevent nausea and vomiting, to help withvacantof the stomach in people with delayed stomach emptying, and to help with gastroesophageal reflux disease. It is also used to treat migraine headaches.This study was aimed to the investigation of antimicrobial and genotoxic effects of metoclopramide using different test systems. The Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined by dilution assay using different concentrations of metoclopramide against 2 bacteria (Bacillus subtilis as a gram-positive bacterial strainand Pseudomonas aeruginosa as a gram-negative bacterial strain). Disk diffusion assay were prepared under sterile conditions disks of drugs (three repeat for each concentration) containing three different doses (50, 100 and 150 μg) were prepared. The Mueller Hinton agar plates were incubated at 37oC for 12 hours. In addition, DNA breaking effects of metoclopramide were analyzed on pET22b plasmid. MIC values of metoclopramideagainst Pseudomonas aeruginosa was 2 mg/ml, and MBC values was 5.4 mg/ml and MIC values of metoclopramideagainst Bacillus subtilis was 0.800 mg/ml, and MBC values was 5.4 mg/ml. The results obtained from disk diffusion assay supported that the metoclopramidehas not anti-bacterial effect against Pseudomonas aeruginosa but has low antibacterial effect on Bacillus subtilis. In this research metoclopramide not demonstrated a cleavage activity on pET22b plasmid DNA. These findings showed that metoclopramide did not have significantly genotoxic and antimicrobial effects (on normal bacterial flora). It can be said that metoclopramide does not have a risk for humans

کلیدواژه ها:

Antibacterial effects ، Metoclopramide ، Pseudomonas aeruginosa ، Bacillus subtilis