Magnetic drug delivery system with a smart pH-responsive layered shell for controlled transportation of L-dopa

The new generation of therapeutic systems for cancers focusses on the concept of targeting and slowing the release of drugs to long-term the therapeutic efficiency and reduces the side effects which mainly originate from the high dosage of the drug in the blood. Today, there are many reports on the use of magnetic high surface area nanoparticles with the ability to respond to pH changes of environment. The use of these nanoparticles enables the targeted transfer of the drug to the target tissue. In the present study, we have developed a novel and efficient targeted anticancer drug delivery system based on the use of a magnetic core-shell structure of Fe3O4@CaAl LDH nanospheres as a carrier for L-Dopa in the treatment of Melanoma. The structural properties of Fe3O4@CaAl@LDopa were characterizes using various techniques like XRD, SEM, TEM, EDX, FT-IR, VSM, TGA, XPS and BET. According to the obtained results, Fe3O4@CaAl@LDopa has a uniform core-shell structure with about 120 nm in average size. This hybrid nanocarrier has a high drug loading (52 wt.%) and drug efficiency (71%). Also, in this drug delivery system, the drug release was sensitive to pH changes. The amount of L-Dopa release from Fe3O4@CaAl LDH nanoparticles was much faster in lower pHs (98.5%) which indicate Fe3O4@CaAl@L-Dopa can be used efficiently in the cancerous cells. Therefore, this system can decrease the cytotoxic effects of L-Dopa by selective L-Dopa delivery. Furthermore, the cytotoxic investigation of L-Dopa and Fe3O4@CaAl@L-Dopa using the MTT assay on Mel-Rm cell lines shows the decrease in proliferation ratio of Mel-Rm cell lines as compared with the free L-Dopa and Fe3O4@CaAl LDH. Also, the values of IC50 for Fe3O4@CaAl@L-Dopa (3.8701 μM) was lower than the corresponding values for free L-Dopa (5.48 μM).

کلیدواژه ها:

L-Dopa ، LDH ، Magnetic nanoparticles ، pH-responsive drug carriers ، Melanoma cancer cells