Isolation and characterization of Bacillus bacterium producing protease enzyme from the Sardabroud River, Mazandaran Province

Enzymes are important bacterial-produced compounds that have many industrial uses. So that many applications used in various industries, including the textile industry, pharmaceuticals, leather, detergents, among Bacillus bacteria is one of the most important microorganisms that produce the protease enzyme. In this research separated samples have taken from water and sediment of Sard-abrod river of Mazandaran province. Then separated samples have been identified by biochemical tests. In order to investigate the ability of bacteria to produce protease enzyme strains cultured in the skimmel agar culture medium, after producing a transparent hallow around colonies. Different strains were compared in terms of the production of protease enzymes. Two isolates were selected from the all pure isolates. The optimum production of protease enzyme was measured by isolating at different times and at different temperatures. The highest protease activity for both selected isolates was determined by 24 and 48 hour incubation times and 37°C, respectively. The results of biochemical tests indicate that the isolated bacteria has related to the Bacillus genus, which has the ability to produce protease and hydrolyze casein. Optimum pH for the enzyme was determined by measuring its activity and its stability at various pH values at 37°C.

کلیدواژه ها:

Bacillus bacteria ، Isolation ، Protease enzymes ، Mazandaran province