Synthesis of magnetic carboxymethyl cellulose nanoparticle for immobilization of Laccase enzyme
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 263
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شناسه ملی سند علمی:
BIOCONF20_201
تاریخ نمایه سازی: 28 اردیبهشت 1398
چکیده مقاله:
An efficient and novel immobilization of Laccase has developed using a magnetic hybrid nanostructured material of iron oxide and carboxymethyl cellulose (Fe3O4–CMC) as support. The resultant magnetic hybrid nanostructured material was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). For this purpose, at the first iron oxide nanoparticle was synthesized from the inorganic salts of Fe (II) and Fe (III) by co-precipitation method and then iron oxide nanoparticles modified with carboxymethyl cellulose.Laccase enzyme (0.025 g/ml) loaded into this magnetic hybrid nanostructured material by using 400 mM of NHydroxysuccinimide(NHS) and 20 mM of 1-Ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) as the activator and incubation lasting 24 hr at aqueous state pH of 5.5 and room temperature. Under this optimum condition, the efficiency of laccase immobilization was at least 35%. According to our result reveal that Fe3O4–CMC is an appropriate and applicable matrix for Laccase immobilization and can be used in different processes
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نویسندگان
Kiyanoush Bakhshande
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Ali Taravati
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Maryam Mohajerani
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran