The protective role of glycyrrhizin on ethanoldamaged glial B92 cells

Glycyrrhizin is one of the most important pharmacological compounds of Licorice plant. The purpose of this study was to investigate the protective effects of glycyrrhizin on the B92 neuroglial cells after treatment with ethanol. B92 cells (1×106 cell/ml) were transferred to 96 plates and incubated with glycyrrhizin (0, 5, 10 and 25 nM) for 24 hours. The cells were then mixed for 80 minutes with absolute ethanol at concentrations of 86 μM. Finally, the viability of the cells was evaluated with neutral red (NR) uptake and MTT tests. It was observed that Glycyrrhizin reduces the level of the cell membrane and mitochondrial damages induced by ethanol in a dose-dependent manner.So at a minimum concentration (5 nanomolar), the membrane damage was significantly reduced. .Also, 10 and 25 nM glycyrrhizin significantly reduced the damage to mitochondria performance. Glycyrrhizin can have a protective effect against ethanol-induced damage in B92 cells.

کلیدواژه ها:

Neuroglial B92 cell ، Glycyrrhizin ، Ethanol