Evaluation of a method for cryopreservation of Persian sturgeon (Acipenser persicus)embryos

Vitrification could provide a promising tool for the cryopreservation of fish embryos. In order to achieve successful cryopreservation, several parameters should betaken into account to prepare successful vitrification protocol. In this study, the combination of permeable and non-permeable cryoprotectants was investigated, at 6 hours after fertilization of Acipenser persicus embryos with applying the stepwise-equilibrated vitrifying solution in order to determine the suitable method to increase the survival rate of embryos after freeze and thaw process. According to the results obtained from freeze and thaw of Persian sturgeon embryos at 6 hours after fertilization, it can be said that Persian sturgeon embryo after fertilization stage is more sensitive to cryoprotectants. Furthermore, the hatching rate in the group was methanol is used for freezing is significantly higher than the group DMSO is used. This indicates that Persian sturgeon embryos are more sensitive to DMSO and DMSO can cause higher death rate in this procedure. The present study reports the successful cryopreservation of Persian sturgeon embryos (Acipenser persicus) by vitrification using 5 step vitrifying solution.

کلیدواژه ها:

Vitrification ، Cryopreservation ، Cryoprotectants ، Persian sturgeon ، Embryos