Investigation of the Relationship between Growth Factors of SCF and FLT-3 in the Proliferation of Umbilical Cord Blood CD + 8 Stem

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 385

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شناسه ملی سند علمی:

NSCMRMED03_370

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Stem cells are a cell of high-division cells that aresplit and multiply throughout life. Growth factors of SCF and FLT-3 arenatural molecules with protein or steroid structures that are involved in the growth and differentiation of many cells. The mutation in SCF andFLT-3 disrupts the proliferation of CD8 + stem cells. The purpose of thisstudy was to examine the relationship between S and F growth factors inthe proliferation of cord blood CD8 + stem cellsMethods: Statistical population: In this study, 38 umbilical cord bloodsamples from newborns from Motahari Hospital of Urmia were used.Real-Time PCR technique was used to extract Total RNA to isolate andextract CD8+ stem cells using flow cytometric method by MACS system.Stemline was used for culture. After extraction, SCF and FLT-3 factors wereused for the proliferation of cells on days 5, 10 and 15. After replicationof growth factors for Total RNA, a real-time PCR technique was used andthe data were analyzed by SPSS V.24 software was analyzedResults: The expression of FLT-3 growth factor expression in CD8+ stemcell proliferation on day 5 (during reproduction) (P <0.001) decreasedthe growth trend. The expression of SCF growth factor on day 5 (P =0.014%, 469 ± 281). The frequency of FLT-3 was 10 (0.041 ± 0.041) onday 10 and the frequency of SCF was 10 (019.0 ± 09.29, P = 0.019),which increased on day 5, the incidence of SCF growth factor Day 15(017.4 ± 4.2) and FLT-3 growth factor (15%, P <0.01) were observed atday 15 (P <0.01).Conclusion: Based on the results, growth factors of SCF and FLT-3 in cordblood CD8+ cell proliferation have a significant relationship with cordblood, which can be used as a substitute for non-growth.

کلیدواژه ها:

SCF -FLT-3 Stem Cell-Cord

نویسندگان

Sasan Talaneh

Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran

Zahra Azadkasb

Urmia University of Medical Sciences, Urmia, Iran

Noshin Ensafi

Urmia University of Medical Sciences, Urmia, Iran