Glioma Stem Cells-Derived Microvesicles Promote Tumor Growth Through Modification of Astrocytes

Background and Aim: Glioma stem cells (GSCs) play an important role inglioblastoma prognosis. Several studies indicated the importance of gliomastem cells-derived microvesicles (GSCs-MVs) in the communication between the tumor and its surrounding microenvironment. In this study,we addressed whether glioma stem cells use microvesicles to modulatenormal astrocytes to facilitate their invasion.Methods: Microvesicles were isolated from glioma stem cells usingdifferential ultracentrifugation and characterized by the use ofTransmission Electron Microscopy and Dynamic Light Scattering. Themicrovesicles were co-incubated with normal astrocytes and thencell growth and expression of genes involved in extracellular matrixremodeling were evaluated.Results: We found that co-incubation of normal astrocytes and GSCs-MVs upregulate the Expression of glial fibrillary acidic protein (GFAP),and activate normal astrocytes. Furthermore, our data showed that GSCs-MVs affects the expression of genes involved in tumor invasion andgrowth in normal astrocytes.Conclusion: Cumulatively, our findings indicate that GSC-MVs canfacilitate tumor growth and invasion by the modifying of the normalastrocytes’ phenotype.

کلیدواژه ها:

Glioma stem cells (GSCs) ، Extracellular vesicles ، Glial fibrillary acidic protein