The Effect of Intraovarian Injection of Ckit Positive Cells Derived from Bone Marrow Cells in Ovarian Rejuvenation of Menopause Rats; A Pre-clinical Study

Background and Aim: Today Cell therapy is one of the new therapeuticmethods in medical sciences and fertility related complications such asmenopause. Bone marrow derived c-kit positive cells, have the ability todifferentiate into different cell types. In this study, intra-ovarian injectionof bone marrow derived c-kit positive cells with the aim of ovarianrejuvenation was implemented in the rat model of ovarian failure.Methods: To induce the rat model of menopause, daily injection of4-vinylcyclohexene dioxide (VCD) was performed for 15 days. 75 Wistarfemale rats were randomly divided into 5 groups: Control (IP injection ofsaline for VCD control), VCD free (IP injection of VCD and letting themfree without intervention), Ckit positive (IP injection of VCD and intraovarianinjection of Ckit positive cells), Ckit negative (IP injection of VCDand intra-ovarian injection of Ckit negative), saline injection (IP injectionof VCD and intra-ovarian injection of saline). Histological evaluation andhormonal assay (FSH and E2) continued up to 8 weeks (once every 2weeks). At the end stage, reproduction performance of 3 rats for eachgroup was assessed.Results: Preliminary results from histological evaluation showed animproved follicles counts in the c-kit positive group compared to c-kitnegative and saline groups, whereas more atretic follicles in differentstages were noticed in c-kit negative rats and saline group. Reproductiveperformance assessment after mating revealed a significantly higher birthrates in c-kit positive group compared to c-kit negative, saline and VCDfreegroups.Conclusion: Intra-ovarian injection of Ckit positive derived from bonemarrow cells is a useful approach for ovarian tissue rejuvenation andreproductive performance return. Future studies and clinical trials willhopefully focus on the uses of Ckit positive cells in the restoration ofhuman ovaries.

کلیدواژه ها:

Infertility ، Menopause ، CKit positive ، Bone marrow cells ، Ovarian rejuvenation