The Role of TiO2 Nano-Tubes in Treatment of Neuronal Cancer

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 335

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شناسه ملی سند علمی:

NSCMRMED03_352

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Titania (TiO2) has been demonstrated as anexcellent candidate for local and targeting drug delivery systems owingto its chemical stability, photocatalytic activity good biocompatibilityand low cost. The common drawbacks of conventional cancer treatmentsare non- targeting, toxicity leading to limiting the amount of desireddrug, poor biodistribution, and efficacy. For this purpose, we used TiO2biomaterial with the suitable structure as drug carriers for the effectiveand targeted transfer of drugs to target cells. TiO2 nanotube surface withbiocompatible materials containing a chemical group that provides adesirable platform to a conjugate of the drug molecules to substrate. Inthis study, we used TiO2 nanotube for neuroblastoma cancer cell killingunder UV light.Methods: Anatase TiO2 nanoparticles with a diameter 10-25 nm wereused as starting material. Morphological studies were carried outusing transmission electron microscope (TEM), x-ray diffraction (XRD)patterns of the powder samples were recorded on a Philips PW3710diffractometer using CoKα radiation (λ= 1.79Ao) at 40kV and 30mA.Neuroblastoma cell line (C549) was purchased from Pasteur InstituteTehran, Iran. Metabolic activity of neuroblastoma cells lines culturedwas assessed by the (MTT) reduction assay. The cytotoxicity assays wereperformed thrice. Finally, the optimum dose of nanotubes was chosenfor cell studies according to the MTT results. The cells were incubatedwith RPMI media +15% FBS & 1% pen/strep. They were cultured for aperiod of 5-7 days and divided to control and treatment groups. Cellstreated with TiO2 at a range of concentration of 50-100 ng/mL and 5 μg/mL cisplatin, an anti-cancer drug too.Results: The morphology and structure of the nanotubes were characterizedby TEM images revealed hallow and open ended tubular structures. Theresult of XRD pattern indicates that the TiO2 is completely comprised ofanatase phase (JCPDS 001-0562). Significant peaks were observed at 2λ = 29.44, 44.34, 56.82, 63.49, 65.21 and 74.37 corresponding to (1 01), (1 0 3), (2 0 0), (1 0 5), (2 1 1) and (2 1 3), respectively. The viabilityof cells on control compare to experimental groups was examined withMTT assay in days of 1, 3 and 5 after incubation. The cell survival ratein 3 and 5 days was significant (P<0.001). Cell viability withTiO2 andcisplatin was more significant than cell group with only cisplatin.Conclusion: Results showed the potential of using TiO2 and cisplatinplatforms as nanocarriers to treat cancer. Anatase TiO2 nanotubes canstrongly absorb the UV light, be excited and generate electron-hole pairsand react with OH– or molecular oxygen to form various reactive oxygenspecies (ROS) which lead to the death of the tumor cells

نویسندگان

Alireza Shams

Department of Anatomy, Alborz University of Medical Sciences, Karaj, Islamic Republic of IRAN

Negin Khoshnood

Biomaterials Research Group, Nanotechnology and Advanced Materials, Materials and Energy Research Center (MERC),Meshkin Dasht, Karaj, Islamic Republic of IRAN