The Use of Liposomes as a Vector and a Member of Nanocarriers Group in Order to Transfer the HGF Gent to Stem Cells for the Treatmentof Keratoconus

Background and Aim: Today, eye cornea is one of the most commondiseases in the eye. It usually occurs in the age group of 10 to 25 years.The glasses or soft lenses that can be used in mild forms of the disease.However, with thinning and deformation of the cornea, this treatment isreplaced by hard lens next treatment is corneal transplantation, whichallows rejection of the transplant to the patient s life. Due to the presenceof hereditary. We present the method of research and treatment of thestratum (Stroma). In this method, we tried to use the liposome to transferthe hgf gene to the nucleus Fat-derived stem cells that are compatiblewith the body and have no side effects. Diagnostic techniques A devicecalled slit lamp is detectable by the keratoconus ophthalmologist in themedium to advanced stages, but in the early stages of the disease withtools such as keratotomy and topography Determining the corneal plane(purity of the cornea) can be identifiedMethods: The use of liposomes as a vector and a member of thenanocarrier group is used to transfer the hgf gene to the nucleus offetal-derived stem cells in the treatment of keratoconus. Hgf is used asa growth factor for growth and debridement of cells. Mesenchymal stemcells due to their ability Differentiating into different cell types is a goodoption for cell therapy and gene transfer in the disease. In this method,we first introduce the hgf gene through the transfection process intoliposomes and then transfer these nanocarriers to the mesenchymal stemcell culture medium Derived from fat are added as a vector of the geneto stem cells They import. Subsequently, by injecting these cells into theophthalmic layer, the cells express the hgf gene and secrete a protein thatdestroys the opaque cells and restores the tissueResults: Nowadays, Most Researches and New Therapeutic MethodsUse Nanotechnology and Stem Cells. In this research, liposomes wereused to transfer the hgf gene to the stem cells nucleus. The stem cellsare then inserted into the eye tissue through the drop. Next, stemcells enter the eye tissue through the droplet and the stem cells treatkeratoconus. Keratoconus is a disease that causes the cornea to thinout. Usually, surgery, laser, and lens are used to treat the disease, whilethey have complications. But the non-invasive drops of cells do not havecomplications.Conclusion: Nowadays, research on nanotechnology and stem cells andregenerative medicine is very important. We envision that this approachmight be an effective treatment modality.

کلیدواژه ها:

Keratoconus ، Liposome ، hgf gene ، Stem cells