Human Placenta Mesenchymal Stem Cell Accelerates Wound Healing in Mice

Background and Aim: Skin is one of the large human body organs thatapproximately consists 5.5% of total body weight in an average 70kg healthy adult human male. The function of the skin is to maintainhemostasis of the internal environment and protects the body against theexternal environment. Any injury or illness that causes disruption in skinintegrity may result in significant disability or even death. The purpose ofthis study was to investigate the effect of transplanted human placentamesenchymal stem cell (hPMSCs) on wound healing.Methods: The full-thickness cutaneous wounds were created by anincision in the dorsal skin of BALB/c mice and treated with hPMSCsaround the wound intradermally. The rate of wound healing wasevaluated by histologic analysis. A total of 20 BALB/c mice, 2 woundsper animal, which were divided into 4 groups were examined in thisstudy. The skin tissues were alternately harvested after wound creation.The harvested tissue sections were stained with hematoxylin and eosin(H&E) and Masson’s trichrome (MT).Results: The histomorphometric analysis revealed re-epithelializationwas minimum in the negative control group and the defect area wasmostly filled with immature granulation tissue (P < 0.05). The best reepithelializationwas detected in the MSCs incorporated Matrigelgroup. MT staining showed that among the experimental groups, MSCsincorporated matrigel induced the greatest collagen synthesis. In contrast,the rate of collagen fiber synthesis and deposition in the wound areawere the lowest in the negative control group followed by the Matrigelgroup.Conclusion: Our results demonstrated that hPMSCs can increase woundhealing rate compared to the control group. Our data showed thathPMSCs are a promising cell source for the treatment of skin wounds.hPMSCs suppress inflammation in the wound and prepare the woundenvironment to heal and accelerate collagen and skin layers formationwithout any immunologic response induction in the genetically unrelateddonor.

کلیدواژه ها:

Human placenta mesenchymal stem cell ، Matrigel ، wound ، Skin ، Cell therapy